The best way to prevent neonatal isoerythrolysis is to perform blood typing with cat red blood cells (phenotype). Due to the monoclonal antibodies that have been developed by Alvedia, this test is very accurate. It is highly recommended to determine whether or not the cat is a b allele carrier either by using a molecular technology (PCR) done with a drop of blood or a bucchal swab of the cat. Up to now, the b allele determination is 100% accurate. The combination of both of these techniques will permit the breeder to know the blood group of the cat’s offspring and whether or not it is a b carrier.
According to the art of science it is very difficult to detect the ab genotype. Thus, the combination of both tests (phenotype and genotype b) is the only way to accurately determine the presence of the ab genotype. Therefore, due to the difficulty in determining the ab genotype, it is recommended that both tests be done in order to determine the exact genotype of the cat.
If you perform only the DNA testing it is impossible to distinguished between a type A group versus a type AB group because this genetic test identifies only the recessive b allele. This b allele is associated with the B phenotype: cats with 2 copies of the b allele have the B blood group and cats with 1 copy of the b allele can have the A blood group or AB blood group.