Alvedia

Collection in a few steps :

1. Prepare intervention :
   • shaving : jugular vein is the most common punction site
   • cleaning : use of chlorhexidine is recommended
   • disinfection
2. Apply anaesthetic gel
3. 3 people required:
   • one to restrain the donor
   • one to sample blood
   • one to handle the collecting bag
4. The patient is restrained in a sitting position or in lateral recumbency.
5. Clamp the tubing using a hemostatic clamp (does not damage the tubing) or a coupe tubulure in order to avoid anticoagulant to go up and out of collection bag.
6. Wear gloves (sampler).
7. Compress jugular groove.
8. Stretch skin in order to locate vein clearly.
9. Insert needle with the tip pointing cranially and withdraw blood by puncturing vein frankly.
10. As soon as blood enters tubing, remove clamp. If blood does not come, then tubing has to be clamped again, needle withdrawn and puncture done again.
    • Hold needle in vein on 1,5 cm
    • Hold tubing manually against table or at root of needle against skin.
    • Hold collection bag inclined from patient
11. Agitate collection bag (manually or using agitator) so that blood and anticoagulant mix together.
12. Once collection bag reaches required weight (477 +/- 48g), stop compression, clamp tube close to needle. Remove needle and put cap back on.
13. Apply compress on puncture site and if needed, bandage for 30 minutes.
14. Push blood present in tube into the collection bag.
15. Seal tube close to the bag.
16. Label collection bags indelibly: date and time of collection, expiration date, type of animal, name of donor, blood group.

NB: in case the animal moves and the needle slips out of vein, either push needle forward or remove it and start the puncture again.

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