Description

Presentation
A Direct Antiglobulin Test (DAT) or Coombs Test is performed to detect the presence of antibodies against red blood cells. It is used in the diagnosis of Immune- Mediated Hemolytic Anemia (IMHA).

Our technology
Our equine DAT Gel Test (or Coombs Test) is based on an individual Gel Test microtube filled with a mixture of gel and specific equine Antiglobulin reagent that will detect the presence of immunoglobulins (IgG & IgM) and/or C3 components binding to the red blood cells surface. A positive result indicates an in vivo sensitization with the presence of auto-immune antibodies.

MATERIAL
 

 

MATERIAL PROVIDED:

 

  • 1 Box of 10 gel Tests
  • 1 Buffer solution

GEL TEST PRODUCTS REQUIRE THE SPECIFIC CENTRIFUGE HETTICH EBA 270 OR DRUCKER TRUE BOND.

DO NOT PURCHASE THE CENTRIFUGE BEFORE ORDERING THE GEL TEST PRODUCTS.

Please contact us at contact@alvedia.com for more information regarding the purchase of the specific centrifuge.

 

 

AN AUTOCONTROL GEL TEST SHOULD BE PERFORMED AT THE SAME TIME AS EACH EQUINE DAT GEL TEST.

MATERIAL PROVIDED:

  • 1 Box of 10 gel Tests
User's guide
RESULTS
PRODUCT INFORMATION

Equine Gel Test DAT – GT-DAT-E-10 – Box of 10 Gel Test DAT Equine

Movie prodcedure

Limitations

Limitations:

  • If the blood tube is hemolyzed OR more than 72 hours: wash 1 time in PBS or saline buffer (NaCl 0.9%) to obtain washed pRBCs.

  • Do not use Gel Test tubes which show signs of drying.

  • Gel Test tubes which show air bubbles or gel drops in the upper part of the tube must be centrifuged before use.

  • Strict adherence to the procedures and recommended equipment, especially the Hettich EBA270 and Drucker True Bond, is essential for a reliable and validated result.

  • A non-specific centrifuge (fixed angle centrifuge) will give you false positive results.

  • Debris, fibrin residues or other artefacts may cause a few unagglutinated cells to trap on top of gel, but these should be interpreted as negative.

  • Use of suspension solutions others than the provided one may modify the reactions.

  • Too diluted or concentrated red blood cell suspensions can cause aberrant results.