Description

Presentation

Dogs have many blood types on the surface of their red blood cells (DEA1, 3, 4, 5, 7, DAL…) With the absence of monoclonal antibodies for all of these blood groups (except for DEA 1), it is mandatory to perform a reliable Crossmatch test (XM) before any transfusion. Crossmatching aims to establish a serological compatibility between the recipient and the donor to detect the presence of alloantibodies.

Our technology

Our canine XM Gel Test (major and/or minor) is based on an individual Gel Test microtube filled with a mixture of gel and specific canine Antiglobulin reagent that will detect the presence of immunoglobulins (IgG & IgM) and/or C3 components binding to the red blood cells surface. Our Gel Test XM Test will allow you to pick-up incompatibilities across all canine blood groups (DEA1, 3, 4, 5, 7, DAL…).

MATERIAL

 

MATERIAL PROVIDED:

  • 1 Box of 10 gel Tests
  • 1 Buffer solution

GEL TEST PRODUCTS REQUIRE THE SPECIFIC CENTRIFUGE HETTICH EBA 270 OR DRUCKER TRUE BOND.

DO NOT PURCHASE THE CENTRIFUGE BEFORE ORDERING THE GEL TEST PRODUCTS.

Please contact us at contact@alvedia.com for more information regarding the purchase of the specific centrifuge.

User's guide
RESULTS
PRODUCT INFORMATION
Canine Gel Test Crossmatch  – GT-XM-C-10 – Box of 10 Gel Test XM Canine
Movie prodcedure

SCIENTIFIC INFORMATION & limitations

 Scientific information:

  • This test allows you to detect all DEA incompatibilities, including DEA 1, DEA 4, DEA 7… and other red blood cell alloantibodies.
  • This test is used to detect alloantibodies after a mismatched transfusion or rare naturally occurring alloantibodies.
  • Blood typing for DEA 1 is always recommended before any transfusion.
  • Positive XM reactions may be observed in IMHA patients due to circulating autoantibodies present in the recipient plasma.
  • Test results must be interpreted in conjunction with the clinical and therapeutic context.

Limitations:

  • Blood bag segment stored for more than 7 days: wash 1 time in PBS or saline buffer (NaCl 0,9%) to obtain washed pRBCs.
  • Do not use Gel Test tubes which show signs of drying.
  • Gel Test tubes which show air bubbles or gel drops in the upper part of the tube must be centrifuged before use.
  • Strict adherence to the procedures and recommended equipment, especially the Hettich EBA270 and Drucker True Bond, is essential for a reliable and validated result.
  • A non-specific centrifuge (fixed angle centrifuge) will give you false positive results.
  • Debris, fibrin residues or other artefacts may cause a few unagglutinated cells to trap on top of gel, but these should be interpreted as negative.
  • Use of suspension solutions others than the provided one may modify the reactions.
  • Too diluted or concentrated red blood cell suspensions can cause aberrant results.